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. Author manuscript; available in PMC: 2009 Aug 26.
Published in final edited form as: Int J Radiat Biol. 2007;83(11-12):761–767. doi: 10.1080/09553000701633135

Figure 1.

Figure 1

Doxycycline concentration dependent expression of cleavable and non-cleavable integrin α6. (A) PC3N-α6-WT and PC3N-α6-RR cells were treated with 0.00001 – 0.01 μg Doxycycline per ml of media. Cell lysates were analyzed for expression levels of integrin α6. The α6 integrin was detected in whole cell lysate using the AA6A rabbit polyclonal antibody on a Western blot. Actin was used as a loading control. (B) PC3N-α6-WT and PC3N-α6-RR cells were left untreated or treated 0.001 μg/ml Doxycycline alone or with 20 μg/ml uPA and the lysates analyzed by PAGE. The integrins α6 (140 kDa) and α6p (~75 kDa) were detected using the AA6A rabbit polyclonal antibody by Western blot analysis.