Table 1.
Oligonucleotide primer sequences used
| Oligonucleotide primer | Sequence (5′→ 3′) |
|---|---|
| Ap_GCLC_PRl | CTCAAAGTCTGTCAATTGGACCT |
| GeneRacer 5′primer | CGACTGGAGCACGAGGACACTGA |
| Ap_GCLC_PFl(rc) | GGTCTGAACTCAACTCTCCATCCAAT |
| GeneRacer 5′ nested primer | GGACACTGACATGGACTGAAGGAGTA |
| M 13 forward primer | GTAAAACGACGGCCAG |
| M 13 reverse primer | CAGGAAACAGCTATGAC |
| Ap_GCLC_Fl | CTTGGATCGCCTCTTTCTTG |
| GeneRacer 3′ primer | GCTGTCAACGATACGCTACGTAACG |
| Ap GCLC F3 | ACGCAAAATTCCAACCACTTTG |
| GeneRacer 3′ nested primer | CGCTACGTAACGGCATGACAGTG |
| Ap_GCLC_PF2 | ATCCAGACTACAAGCAGGACTCG |
| Ap_GCLC_qFl | TGCGCGAAAGGATAAGTTCTACT |
| Ap_GCLC_qRl | CAGACTCACAGCACTCTGAATGG |