Table 2. Variability in the amplified Streptococcus pneumoniae 16S rDNA sequencesa.
| Pleural fluid | Cerebrospinal fluid | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| XEB44b | XMN22 | XCA73c | Bacterial isolates | Ref. straind | ||||||||||||||
| Specimen 1- PCR analysis 1 | Specimen 1-PCR analysis 2 | Specimen 1-PCR analysis | Specimen 1-PCR analysis 1 | Specimen 2-PCR analysis 1 | ||||||||||||||
| Corresponding E. coli 16S rDNA position | Original PCR product | Clones from PCR products | Clones from PCR products | Clones from PCR products | Original PCR product | Clones from PCR products | Original PCR product | Clones from PCR products | ||||||||||
| A | B | C | D | E | F | G | H | I | J | K | SF10014 | SF10175 | SF10314 | |||||
| 120 | A/G | G | G | A | A | A | A | A | A | A | A | A | A | A | A | A | A | A |
| 260 | A/G | G | A | G | G | A | G | G | G | G | G | G | G | G | G | G | G | G |
| 273 | A | A | A | A | A | A | G | A | A | A | A | A | A | A | A | A | A | A |
| 279 | A | A | A | A | G | A | A | A | A | A | A | A | A | A | A | A | A | A |
| 442 | T | T | T | T | C | T | T | T | T | T | T | T | T | T | T | T | T | T |
aNucleotides in bold type differ with the reference strain sequence at the indicated rDNA position. bOne pleural fluid sample was analyzed with PCR on two occasions. cTwo cerebrospinal fluid specimens were taken 5 days apart from the same patient. dPCR = polymerase chain reaction; E. coli = Escherichia coli. eStreptococcus pneumoniae NCTC 7465T (GenBank accession no. AJ001246).