Table 1. Reverse transcriptase-polymerase chain reaction (RT-PCR) primers used to sequence Eastern equine encephalomyelitis virus RNA.
Title | Sense | Primer | Use | Region |
---|---|---|---|---|
9930 | Forward | 5´- GCGCTGCTTATTTTGCTGT -3 | RT-PCR | E1 |
11582 | Reverse | 5´- ATTATGCGCTGCCTGTAGTGTTTA –3´ | RT-PCR | E1 |
4043 | Forward | 5´- GTGCGCGGCGACATAACAAAGAG- 3´ | RT-PCR | NSP3 |
4976 | Reverse | 5´- CCGGGGGTACAGTGCCAGAGAA –3´ | RT-PCR | NSP3 |
10470 | Forward | 5´- ATGCCAAACTCATCATAGGTCCACT –3´ | Sequencing | E1 |
10938 | Forward | 5´- GTATAGCCACCGTTGCCTACAAATC –3´ | Sequencing | E1 |
11345 | Forward | 5´- CAGGCAGTGTATAAGGCTGTCTTAC –3´ | Sequencing | E1 |
T3 | Forward | 5´- AATTAACCCTCACTAAAGGGA –3´ | Sequencing | NSP3 |