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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Gene. 2009 May 27;444(1-2):1–9. doi: 10.1016/j.gene.2009.05.010

Figure 6. MED1 positively regulates Tnk1/Kos1 transcription.

Figure 6

Identification of the nuclear proteins that bind to the MED1 consensus binding site (A). The protein-DNA complex formed between 32P labeled Tnk-MED1 and NIH3T3 nuclear extract (lane1) is competed with 100 to 200 fold molar excess of unlabeled Tnk-MED1 or an oligonucleotide containing the MED1 consensus site. Effect of serum or Leukemia inhibitory factor (LIF) withdrawal on the protein-DNA complex formation (B). 32P labeled Tnk-MED1 or 32P labeled Tnk38 was incubated with nuclear extract prepared from NIH 3T3 cells in the presence of serum (lanes 1,5) and in the absence of serum (lane 2,6) or nuclear extract prepared from CCE cells d0 (lane3) and d3 (lane 4) of LIF withdrawal.