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(A) HPLC chromatograms showing the thionicotinamide base-exchange reaction and negative control. (B) Steady-state saturation kinetics of the thionicotinamide base-exchange reaction as catalyzed by Pf-Sir2 in the presence of 400 ∝M NAD+ and 400 ∝M H3 peptide and increasing concentrations of thionicotinamide.
