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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Biochemistry. 2008 Aug 26;47(38):10227–10239. doi: 10.1021/bi800767t

Figure 4. Hydrolysis of NAD+ Catalyzed by Pf-Sir2.

Figure 4

(A) HPLC chromatograms showing the production of ADPR by SirT1 and Pf-Sir2 under similar conditions. (B) the kinetics of the hydrolysis reaction for varying concentrations of peptide carried out in the presence of 400 ∝M NAD+ and 150 mM phosphate buffer, pH 7.3.