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. Author manuscript; available in PMC: 2009 Aug 26.
Published in final edited form as: Neuron. 2008 Dec 26;60(6):1068–1081. doi: 10.1016/j.neuron.2008.10.046

Figure 1.

Figure 1

Expression of the teto-M71-IRES-tau-lacZ transgene (M71 Tg) in olfactory sensory epithelia. (A) Schematic of the genetic strategy to express the M71 odorant receptor in all olfactory sensory neurons. The transgene teto-M71-IRES-tau-lacZ can be activated in all sensory neurons by the expression of tTA from the OMP-IRES-tTA locus. (B) Expression of OMP-IRES-tau-lacZ (detected by x-gal staining, blue) marks olfactory sensory neurons in a whole mount preparation in: the main olfactory epithelium (MOE), septal organ (SO), and vomeronasal organ (VNO), as well as in axons of sensory neurons from these areas as they project to the main (OB) and accessory olfactory bulb. (C) The expression of the M71 transgene in all the sensory epithelia detected by x-gal staining (blue) of a whole mount preparation. (D) Expression of the M71 transgene across all zones of the MOE as detected by x-gal staining in a whole mount preparation. (E) Immunohistochemical detection of the expression of lacZ (green) in coronal sections through the olfactory epithelium of control, OMP-IRES-tau-lacZ mice, counterstained with Toto-3 (blue). (F-H) Immunohistochemical detection of the expression of lacZ and M71 in coronal sections through the main olfactory epithelium of M71 transgenic mice. (F) Staining with antibody to lacZ (green). (G) Staining with antibody directed against the M71 receptor (red). (H) Merged fields of F and G. Nuclei are counterstained with Toto-3 (blue). (I) Immunohistochemical detection of the expression of lacZ (green) in coronal sections through the VNO of control OMP-IRES-tau-lacZ mice, counterstained with Toto-3 (blue) (J-L) Immunohistochemical detection of the expression of lacZ and M71 in coronal sections through the VNO of M71 transgenic mice. (J) Staining with antibody to lacZ (green). (K) Staining with antibody directed against the M71 receptor (red). (L) Merged fields of J and K. Nuclei are counterstained with Toto-3 (blue). (M-O) Two-color RNA in situ hybridization detects the ubiquitous expression the M71-IRES-tau-lacZ transgene and the OMP gene in sections through the olfactory epithelium of M71 transgenic mice. (M) RNA in situ hybridization with antisense probe to lacZ RNA (green). (N) RNA in situ hybridization with antisense probe for OMP RNA (red). (O) Merged fields of G and H with nuclei counterstained by Toto-3 (blue).