(A–C) In situ expression pattern of SPL3.
(A) 22 day-old vegetative shoot apex hybridized with a sense strand control.
(B) 15-day-old vegetative shoot apex hybridized with antisense probe.
(C) 22-day-old vegetative shoot apex hybridized with antisense probe. The abundance of SPL3 mRNA increases with time.
(D–F) In situ expression pattern of SPL9; all samples hybridized with an antisense probe.
(D) 22-day-old vegetative shoot apex of an RNA-null allele of SPL9.
(E) 22-day-old wild-type vegetative shoot apex.
(F) 22-day-old vegetative shoot apex from a plant expressing a miR156-insensitive SPL9 genomic sequence under the control of the SPL9 promoter. SPL9 is expressed in young leaf primordia.
(G) Four-week-old rosettes of wild-type, mutant and transgenic lines of Arabidopsis grown in short days. rSPL3 = 35S∷rSPL3, rSPL9 = pSPL9∷rSPL9, rSPL10 = rSPL10∷rSPL10.
(H) The shape and abaxial trichome phenotypes of fully expanded leaves of wild-type, spl9-4, and transgenic lines expressing miR156-resistant forms of SPL3, SPL9 and SPL10. These genes promote different adult traits. Asterisks indicate significant difference from wild-type (P <0.01, n= 18, ± SD).