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. Author manuscript; available in PMC: 2010 Aug 21.
Published in final edited form as: Cell. 2009 Aug 21;138(4):750–759. doi: 10.1016/j.cell.2009.06.031

Figure 2. SPL3, SPL9 and SPL10 have diverse roles in vegetative development.

Figure 2

(A–C) In situ expression pattern of SPL3.

(A) 22 day-old vegetative shoot apex hybridized with a sense strand control.

(B) 15-day-old vegetative shoot apex hybridized with antisense probe.

(C) 22-day-old vegetative shoot apex hybridized with antisense probe. The abundance of SPL3 mRNA increases with time.

(D–F) In situ expression pattern of SPL9; all samples hybridized with an antisense probe.

(D) 22-day-old vegetative shoot apex of an RNA-null allele of SPL9.

(E) 22-day-old wild-type vegetative shoot apex.

(F) 22-day-old vegetative shoot apex from a plant expressing a miR156-insensitive SPL9 genomic sequence under the control of the SPL9 promoter. SPL9 is expressed in young leaf primordia.

(G) Four-week-old rosettes of wild-type, mutant and transgenic lines of Arabidopsis grown in short days. rSPL3 = 35S∷rSPL3, rSPL9 = pSPL9∷rSPL9, rSPL10 = rSPL10∷rSPL10.

(H) The shape and abaxial trichome phenotypes of fully expanded leaves of wild-type, spl9-4, and transgenic lines expressing miR156-resistant forms of SPL3, SPL9 and SPL10. These genes promote different adult traits. Asterisks indicate significant difference from wild-type (P <0.01, n= 18, ± SD).