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. Author manuscript; available in PMC: 2010 Aug 21.
Published in final edited form as: Cell. 2009 Aug 21;138(4):750–759. doi: 10.1016/j.cell.2009.06.031

Figure 3. miR172 acts downstream of miR156.

Figure 3

(A) Blot of small RNA from the shoot apex of wild-type plants of different ages hybridized sequentially with probes to miR156 and miR172. The levels of these miRNAs change in a complementary fashion. U6 served as a loading control.

(B) Blots of small RNA from 35S∷miR156a 35S∷miR172b (14-day-old) and 35S∷MIM156 (20-day-old) plants hybridized sequentially with probes to miR156 and miR172. miR156 represses miR172. U6 was used as loading control.

(C) Leaf shape and abaxial trichome phenotypes of fully expanded rosette leaves of wild-type, 35S∷miR156a 35S∷miR172b and 35S∷miR156a 35S∷miR172b double transgenic plants. 35S∷miR172b partially rescues the 35S∷miR156a over-expression phenotype.

Numbers indicate fold change relative to wild-type. Asterisks indicate significant difference from wild-type (P <0.01, n=18 plants, ± SD).