Figure 7. Effect of GIT1 on EC tube formation.
HUVEC were transfected with control siRNA or GIT1 siRNA. After serum starvation for 24 h, the cells were seeded on 6-well plates at 1×105 cells/well precoated with Matrigel and maintained in serum free medium, or with VEGF (50 ng/ml). Tube formation was visualized after 6 h (A) and analyzed using ImageJ (B).