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. Author manuscript; available in PMC: 2010 Jul 21.

Published in final edited form as: Langmuir. 2009 Jul 21;25(14):8131–8143. doi: 10.1021/la8039434

Fig. 2 — (A) Surface density, Γ, versus surface pressure, Π, for several bulk concentrations of albumin and (B) surface density, Γ, (Δ) and surface pressure, Π, () versus trough area of 10 mg/mL albumin in buffer (NaCl 150mM, NaHCO₃ 0.2mM and pH=7.0) at room temperature (22°C) compressed on a Langmuir trough. Dashed lines in (A) show the Langmuir equation of state where the close-packed surface density is Γ_∥ for molecules lying parallel to the air-water interface and Γ_┴ for molecules lying perpendicular. The solid line in (A) is a linear fit to the Γ(Π) data of 2mg/mL albumin.

Inline graphic — (A) Surface density, Γ, versus surface pressure, Π, for several bulk concentrations of albumin and (B) surface density, Γ, (Δ) and surface pressure, Π, () versus trough area of 10 mg/mL albumin in buffer (NaCl 150mM, NaHCO₃ 0.2mM and pH=7.0) at room temperature (22°C) compressed on a Langmuir trough. Dashed lines in (A) show the Langmuir equation of state where the close-packed surface density is Γ_∥ for molecules lying parallel to the air-water interface and Γ_┴ for molecules lying perpendicular. The solid line in (A) is a linear fit to the Γ(Π) data of 2mg/mL albumin.