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. Author manuscript; available in PMC: 2009 Dec 23.
Published in final edited form as: Biochemistry. 2008 Dec 23;47(51):13699–13710. doi: 10.1021/bi801507s

Figure 5. NBD-CL and etoposide inhibit H2O2-dependent oligomerization of cyt c.

Figure 5

Typical Western blots of cyt c and its oligomeric products after staining with anti-cyt c antibody. Cyt c (1.5 μM) was pre-incubated with liposomes (concentration of phospholipids 25 μM) in HEPES buffer (20 mM, pH 7.4) for 15 min, and then aliquots of H2O2 (at a final concentration of 25 μM) were added every 15 min during 1 h interval at 37°C. Liposomes were prepared from DOPC/TOCL (1:1). Mol % of NBD-CL in liposomes and etoposide concentration were as shown above the gel.