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IL6 production by early-life SES. PBMCs were cultured for 24 h with ligands against TLRs. After supernatants were harvested, the proinflammatory cytokine IL6 was measured by ELISA. Low early-life SES was associated with increased production of IL6 in cultures stimulated with (A) the TLR3 ligand poly(I:C) (P = 0.03) and (B) the TLR5 ligand flagellin (P = 0.05).
