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Two-photon fluorescence imaging of H. pylori in PGM. H pylori (stained with BacLight) in PGM with 5 mM urea, prepared with BCECF and driven to an initial pH of 2 by HCl (A), immediately after sample preparation (Scale bar, 10 μm) (B), at 3 min following preparation, and (C), at 27 min following preparation. A plot of the corresponding rise in BCECF fluorescence intensity over time is shown in D.
