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. 2009 Aug 25;106(34):14536–14541. doi: 10.1073/pnas.0907560106

Fig. 1.

Fig. 1.

AE sensitivities of MCF-7 monoclonal sublines. (A) Phase-contrast images of the original MCF-7 cell culture and its monoclonal sublines isolated in the presence of Fv: 40 nM (F40–3, -6, -7) or 100 nM (F100–1, -3, -7, -16). (B and C) Drug resistance of Tam- or Fv-selected sublines. Cells were exposed to Fv (0.1 μM), 4-hydroxyTam (1 μM), paclitaxel (1 μM), or vehicle (0.1% ethanol) for 10 days, followed by visualization (B) and quantitation (C) of survived cells by sulforhodamine-B staining. Survived cell numbers relative to vehicle controls (mean ± SEM) are shown for individual sublines (open symbols) and each group (closed symbols). (D) AE-induced activation of caspase-7. Relative numbers of DEVDase-positive cells (mean ± SEM, n ≥ 3) after 48-hour exposure to 0.1 μM Fv (open symbols) or vehicle (closed symbols) are plotted. Small symbols represent individual sublines. Large symbols represent groups. (E) Cell cycle arrest by Fv. Unselected, Tam-selected, and Fv-selected sublines were exposed to Fv (0.1 μM) or vehicle (0.1% ethanol) for 48 h. Symbols indicate the relative S-phase cell population in cultures exposed to vehicle (open symbols) or to Fv (closed symbols). All data indicate the mean of at least 3 independent assays whose SEs were not greater than 10% of the means.