Table 2.
Parameters extracted from fits to the Hill equation
| Terminase | Vmax, bp/s | Km, μM | Vmax/Km, bp·s−1·μM−1 | Hill coefficient |
|---|---|---|---|---|
| WT | 580 | 33.8 ± 8.6 | 17.1 ± 4.3 | 0.75 ± 0.15 |
| Y46F | 402 | 22.2 ± 2.1 | 18.3 ± 1.7 | 1.17 ± 0.13 |
Vmax is measured from force clamp measurements and is imposed for fits. Fits without imposing a Vmax constraint for WT and Y46F, while adding more uncertainty to Km, similarly yield Hill coefficients ≤1. Velocities at concentrations <10 μM for Y46F were not measured because of the low efficiency of initiation at low ATP concentrations.