Fig. 4.

Physiological relevance of the Nup120–Nup133 interaction. (A) Detection of poly(A)⁺ mRNA using an Alexa-647 labeled 50-mer oligo dT FISH probe (Top). Wild-type cells (Left) display a diffuse FISH signal, while nup120Δ (Middle) and nup133Δ (Right) cells yield strong nuclear signals that coincide with DAPI staining, consistent with poly(A)⁺ mRNA retention inside the nucleus. (B) Quantitation of nuclear poly(A)⁺ mRNA retention in nup120Δ and nup133Δ yeast strains complemented with various Nup120 and Nup133 variants. The percentages refer to the fraction of cells that displayed marked nuclear staining and are derived from 3 independent experiments.