Fig. 3.

Dissection of acute permeability versus neutrophil infiltration in Akt1^−/− mice. The Akt1^−/− mice display a marked reduction in (A) edema formation, (B) neutrophil infiltration, and (C) NO₂⁻ production in air pouch inflammation induced by carrageenan (1% wt/vol) compared with those of the WT control. (D) Twenty-four hours after intrapouch injection of carrageenan, neutrophils are recovered and evaluated for COX-2 and inducible nitric oxide synthase expression by Western blot analysis (n = 3, per group). The carrageenan air pouch (4 h) in chimeric mice did not show differences in edema formation, neutrophil accumulation, or NO₂⁻ levels between WT mice engrafted with WT (open bar) and Akt1^−/− (filled bar) bone marrow or between Akt1^−/− mice engrafted with WT (gray bar) and Akt1^−/− (dotted bar; n = 5 per group). The data shown represent the mean ± SEM. *, P < 0.05; **, P < 0.01