Fig. 5.

Knocking down USP33 eliminates Slit activity in suppressing SDF1-induced chemotaxis. Dunn chamber chemotaxis assay was performed as described for Fig. 3, except that MDA231 cells were transfected with control or USP33-specific siRNAs. (A) Distribution of mock- or Slit2-treated cells under SDF1 gradients after transfection with the corresponding siRNA. (B) Representative trajectories of seven migrating cells expressing control siRNA or siUSP33, in the presence of the control or Slit2 under SDF1 gradients. (C and D) Quantitative analyses are shown in C (migration speed) and D (FMI). Data are presented as the mean ± SEM (n = 30 from three independent experiments; 10 cells per experiment). ***, P < 0.001 by Mann–Whitney test.