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•NO-enhanced binding of nuclear factors to the ARE of the NQO1 gene. DIG-end-labeled ARE of the human NQO1 gene was incubated with nuclear extract from •NO-treated HCT116 cells as described in Materials and Methods. Mobility-shifted ARE–nuclear protein complex is indicated by the arrow. To verify specificity of binding, unlabeled ARE and 125-fold excess of unlabeled ARE plus labeled ARE were added in a competition assay.
