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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Mol Immunol. 2009 Jul 9;46(15):3009–3017. doi: 10.1016/j.molimm.2009.06.013

Figure 2.

Figure 2

Runx1 binds separate sites upstream of Dβ2. Nuclear extracts from the P5424 cell line were incubated with radiolabeled ds oligonucleotide probes to the putative Runx binding sites: R-I (lanes 1-7) and R-II (lanes 8-14). Probes were incubated with nuclear extract alone (lanes 1 and 8), in the presence of 100-fold molar excess of the indicated unlabeled competitors (lanes 2-5 and 9-12), or in the presence of the indicated Abs (lanes 6-7 and 13-14). Specific nucleoprotein (filled arrows) and Ab-supershifted complexes (empty arrows) are indicated.