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GATA-3 binds multiple sites within 5′PDβ2. Nuclear extracts from the P5424 cell line were incubated with radiolabeled probes to the 4 putative GATA binding sites in 5′PDβ2 as well as an NF-Y control. In each case, reaction mixtures included probe and nuclear extract alone (-), or in the presence of the indicated Abs. Antibody-attenuated (filled arrows) and supershifted complexes (empty arrows) are indicated.
