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Mutational analysis of 5′PDβ2 regulation. Individual transcription factor binding sites within the full-length promoter fragment were selectively mutated to assess their contribution to 5′PDβ2 activity. P5424 transfectants of the indicated mutant flP pGL3-Eβ reporters were assayed for promoter activity. Results from 7 independent co-transfections with pSV-RL were normalized to Renilla levels, and expressed as percent wildtype flP.
