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. 2009 Sep 15;7(9):e1000196. doi: 10.1371/journal.pbio.1000196

Figure 7. Exogenous FGF9 can activate Sox9 transcription in gonads lacking functional MAP3K4.

Figure 7

(A) Culture of a wild-type XX gonad (left) from 11.5 dpc for 48 h in the presence of a bead coated in BSA does not activate Sox9 transcription based on in situ hybridisation analysis. In contrast, a bead coated in FGF9 does result in up-regulation of Sox9 in both XX control gonads (centre) and XY byg/byg gonads (right). The small circle adjacent to each gonad shows the approximate position of the bead during culture. (B) In a separate experiment, FGF9-coated beads again activate Sox9 transcription in wild-type XX and XY byg/byg gonads, but a bead coated in BSA does not activate Sox9 transcription in XY byg/byg gonads. Note the increased size of gonads, both XX and XY, after exposure to exogenous FGF9. All gonads were from embryos on the C57BL/6J background.