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Effects of down-regulation of Gz-alpha protein expression in pituitary cells (A) on ET-1-induced cAMP signaling (B) and PRL release (C). Primary cultures of anterior pituitary cells were treated overnight with Gzα-specific antisense and control missense phosphorothio-modified oligodeoxynucleotides prior to stimulation with 100nM ET-1. cAMP content, PRL release and Gzα expression were later assayed (see text for further details).
