Table 2.
Immunolocalization of Kim-1, RPA-1, and RPA-2 in SD rats treated with Gen or Cis.
| Age and sex (days old) |
Renal lesion scorea (0-5) |
IHC staining intensity for specific immunoreactivityb |
Nonspecific binding in necrotic proximal tubular cells |
||||
|---|---|---|---|---|---|---|---|
| Treatment | Kim-1 | RPA-1 | RPA-2 | RPA-1 | RPA-2 | ||
| 10, 80, ♂ | Saline for Gen | 0 | −, S1/S2,S3 | CL | CL | − | − |
| 10, ♂ | Gen 100 mg/kg/day × 6 days | 2* | ++, S1/S2,S3 | +, CD (cortex) | +, LH | + | + |
| 80, ♂ | Gen 100 mg/kg/day × 6 days | 3.3* | +++, S1/S2,S3 | ++, CD (cortex) | +, LH | + | + |
| 80, ♂ | Gen 100 mg/kg/day × 14 days | 5* | +++, S1/S2,S3 | +++, CD (cortex) | +, LH | + | + |
| 10, 40, 80, ♂ | Saline for Cis | 0 | −, S1/S2,S3 | CL | CL | − | − |
| 10, ♂ | Cis, 1, 3, 6 mg/kg | 0.3 (0-2.0) | +, S3; −, S1/S2 | +, CD (medulla) | +, LH | − | − |
| 40, ♂ | Cis, 1, 3, 6 mg/kg | 2.6* (0.5-4.0) | ++, S; −, S1/S2 | ++, CD (medulla) | ++, LH | − | − |
| 80, ♂ | Cis, 1, 3, 6 mg/kg | 3.25* (1.5-5) | ++, S3; −, S1/S2 | +++, CD (medulla) | +++, LH | − | − |
Abbreviations: CL, constitutive level of RPA-1 or RPA-2 immunostaining intensity; S1/S2, the S1 & S2 segments of proximal convoluted tubules; S3, the S3 segments of proximal straight tubule.
a
For Gen-nephrotoxicity, renal lesion score indicates average score from each treatment group. For Cis-nephrotoxicity, renal lesion score indicates average score from 3 treatment groups followed by reference range of 3 groups (in parentheses).
b
IHC keys: Scores of −, +, ++, and +++ represent no positive reaction, weak, moderate, and strong positive reaction, respectively.
*
Statistically significant different as compared with saline-treated with saline (p < .05).