Skip to main content
. 2009 Sep 4;4(9):e6808. doi: 10.1371/journal.pone.0006808

Figure 2. Leptin Decreases FoxO3a Activity and Increases PGC-1α Protein in Gastrocnemius Muscle.

Figure 2

(A) Representative Western blot analyses of forkhead box class O (FoxO3a) and phospho-FoxO3a proteins of gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice are shown. β-actin was used as a loading control (n = 8 per group). (B) Representative Western blot analyses of Akt activity evidenced by phosphorylated-Akt and Akt proteins ratio of gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice (n = 8 per group). (C) Representative Western blot analyses of AMP-activated protein kinase (AMPK) activity evidenced by phosphorylated-AMPK and AMPK proteins ratio of gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice are shown (n = 5 per group). (D) Representative Western blot analyses of AMP- (PGC-1α) of gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice are shown. β-actin was used as a loading control (n = 8 per group). Data are expressed as mean±SEM. G: genotype, T: treatment.