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. 2009 Sep 4;4(9):e6808. doi: 10.1371/journal.pone.0006808

Figure 3. Leptin Decreases MAFbx and MuRF1 Protein Expression in Gastrocnemius Muscle of Wild Type and ob/ob Mice.

Figure 3

(A) Representative Western blot analyses of muscle atrophy F box (MAFbx) and muscle RING finger 1 (MuRF1) proteins of gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice are shown. β-actin was used as a loading control (n = 8 per group). *P<0.05. (B) Immunostaining for MAFbx and MuRF1 proteins was assessed by optic microscopy. Representative images are shown for gastrocnemius muscle. Scale bars = 50 µm. Data are presented as mean±SEM. G: genotype, T: treatment.