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. 2009 Sep 4;4(9):e6808. doi: 10.1371/journal.pone.0006808

Figure 5. Leptin Enhances Muscle Cell Proliferation.

Figure 5

(A) Immunohistochemical staining for dystrophin and PCNA of gastrocnemious muscle of wild type and ob/ob mice (n = 3 per group). Scale bar = 50 µm. (B) Immunohistochemical staining and relative stained nuclei number for PCNA evaluated among 500 cells in gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice (n = 3 per group). Scale bar = 50 µm. **P<0.01. (C) Immunohistochemical staining and relative stained nuclei number for cyclin D1 evaluated among 500 cells in gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice (n = 3 per group). Scale bar = 50 µm. (D) Immunohistochemical staining and relative stained nuclei number for p27Kip1 evaluated among 500 cells in gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice (n = 3 per group). Scale bar = 50 µm. *P<0.05 and **P<0.01. Data are expressed as mean±SEM. G: genotype, T: treatment.