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. 2009 Sep 4;4(9):e6808. doi: 10.1371/journal.pone.0006808

Figure 6. Leptin Increases the Expression of Contractile Proteins in Skeletal Muscle.

Figure 6

(A) Representative Western blot analyses of myosin heavy chain type I (MHC I), myosin heavy chain type II (MHC II), slow and fast troponin T (TnT) proteins of gastrocnemius muscle of PBS (open), pair-fed (gray) and leptin-treated (closed) wild type and ob/ob mice are shown. β-actin was used as a loading control (n = 8 per group). (B) Immunohistochemical staining for slow and fast troponin T (TnT) of gastrocnemius muscle of wild type and ob/ob mice. Scale bars = 100 µm. Data are expressed as mean±SEM. G: genotype, T: treatment.