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. 2007 Dec 28;18(9):2117–2131. doi: 10.1093/cercor/bhm238

Figure 8.

Figure 8.

D/V patterning and layer V neurons are modified by COUP-TFI dosage. (A–A′) β-Galactosidase staining on coronal sections through the telencephalon of WT;BAT-gal (A), D6/COUP-TFI;BAT-gal (A′), and COUP-TFI–/–;BAT-gal (A′′) at E15.5. β-Catenin activation follows a dorsalhigh–ventrallow gradient that is disrupted in opposing ways in the 2 genotypes (compare arrowheads in A–A′′). (B–D′′) RNA in situ hybridizations on coronal sections through the telencephalon of WT (B–D), D6/COUP-TFI (B′–D′), and COUP-TFI–/– (B′′–D′′) for ER81 (B–B′′), Fezl (C–C′′), and p75 (D–D′′). The D/V expression pattern of these markers is affected by altering COUP-TFI gene dosage: arrowheads show the complementary D/V changes in D6 and COUP-TFI–/– animals (E-F″) RNA in situ hybridizations on coronal sections through the telencephalon of WT (E, F), D6/COUP-TFI (E′-F′), and COUP-TFI−/− (E″-F″) for ER81 (E′E″) and Fez-l (F-F″) at E18.5. COUP-TFI dosage affects milecular markers of different layer V neurons.