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. 2009 Jul;27(7):1654–1665. doi: 10.1002/stem.90

Figure 4.

Figure 4

Expression of HOXA3 in diabetic wounds stimulates mobilization of endothelial progenitor cells (EPCs). (A): Representative images of wound sections from nondiabetic mice (non-db) and diabetic mice (db) treated with control (empty vector) or CMV-HOXA3 plasmid. Upper panels show GFP+ bone marrow-derived cells at day 7 and lower panels show Cd34+ cells. Scale bar = 100 μm. (B): Percentage of total GFP+ cells that are double positive for Cd34 recruited to the wound at day 7 in nondiabetic mice treated with control plasmid, and diabetic mice treated with control (db+cntl) or CMV-HOXA3 (db+A3) plasmid. (C): Representative scatter plots of cleared peripheral blood from diabetic GFP chimeras at day 4 following wounding treated with control (db2+c) or CMV-HOXA3 (db7+A3) plasmid. Cells in the upper right quadrant are GFP+Cd133+Cd34+ (early EPC) cells. (D): Quantification of early EPCs in peripheral blood at day 4 following wounding of control-treated or CMV-HOXA3-treated diabetic wounds. (E): Representative scatter plots of cleared peripheral blood from diabetic GFP chimeras at day 7 following wounding treated with control (db2+c) or CMV-HOXA3 (db7+A3) plasmid. Cells in the upper right quadrant are GFP+Cd133+Cd34+ (early EPC) cells. (F): Quantification of early EPCs in peripheral blood at day 7 following wounding of control-treated or CMV-HOXA3-treated diabetic wounds. Abbreviations: APC, allophycocyanin; db+A3 D7, diabetic wound treated with CMV-HOXA3 at day 7; db+a3 and db+A3, diabetic wound treated with CMV-HOXA3 at day 3; db+C, diabetic wound treated with control; db+cntl, diabetic wound treated with control; db+cntl D7, diabetic wound treated with control at day 7; db1+C, diabetic wound 1 treated with control; db2+C, diabetic wound 2 treated with control; db7+A3, diabetic wound treated with CMV-HOXA3 at day 7; FMO, fluorescence minus one control; GFP, green fluorescent protein; non-db D7, nondiabetic wound at day 7; non db, nondiabetic wound; PB, peripheral blood; PE, phycoerythrin.