Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Aug 24;186(4):481–490. doi: 10.1083/jcb.200902113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Mennella et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Expression of the phosphomimetic KLP10A S573E mutant dampens MT dynamics during interphase S2 cells. (a, top) Interphase cell expressing EGFP–α-tubulin and mRFP-KLP10A wt. (bottom) Magnified images (of the boxed region) from a time-lapse video were used to analyze MT dwell times. Numbers are shown in elapsed time (seconds). Arrows indicate a catastrophe. (b) Western blot of S2 cell lysates treated with control (CTR) dsRNA or KLP10A 5′ UTR dsRNA. (c) Mean dwell times of MT ends in the vicinity of the cell cortex. Cells expressing comparable levels of mRFP-KLP10A were selected for analysis. S573E, n = 200 (10); S573A, n = 200 (11); wt, n = 201 (10); no KLP10A, n = 199 (10); with numbers in parentheses indicating the number of cells analyzed. **, P < 0.01. Error bars indicate SD. Bar, 10 µm.

HHS Vulnerability Disclosure