Fig. 2.

dGal-1-induced exposure of PS on HL60 cells requires expression of complex-type N-glycans. HL60 cells were treated with 10 μM dGal-1 for 4 h, disengaged with lactose, stained with FITC-annexin V and PI, and then analyzed by flow cytometry. The indicated cells were preincubated with kifunensine for 7 days (A) or with benzylGalNAc for 3 days (B). The results are depicted as the percentage of cells that stained with annexin V above a threshold level, but that remained viable as assessed by staining with PI below a threshold level. The data represents the mean ± SD of three experiments.