Fig. 4.

dGal-1-induced exposure of PS on Molt-4 and Jurkat cells requires expression of complex-type N-glycans. Molt-4 cells (A, B) and Jurkat cells (C, D) were treated with 10 μM dGal-1 for 4 h, disengaged with lactose, incubated with a mixture of FITC-conjugated annexin V and PI, and analyzed by flow cytometry. The indicated cells were preincubated with kifunensine for 3 days or with benzylGalNAc for 3 days. The data are depicted as the percentage of cells that stained with annexin V above a threshold level, but that remained viable as assessed by staining with PI below a threshold level. The data represent the mean ± SD of three experiments.