Fig. 5.

dGal-1-induced elevation of cytosolic Ca²⁺ in Molt-4 cells requires expression of complex-type N-glycans. Cytosolic Ca²⁺ levels in stirred Fluo-4-labeled Molt-4 cells were continuously measured in a fluorimeter. (A) Control or BAPTA-loaded Molt-4 cells in a Ca²⁺-containing buffer were incubated with 10 μM dGal-1 in the presence or absence of 20 mM lactose. (B) Kifunensine-treated Molt-4 cells were incubated with 10 μM dGal-1 in the presence or absence of 20 mM lactose. (C) BenzylGalNAc-treated Molt-4 cells were incubated with 10 μM dGal-1 in the presence or absence of 20 mM lactose. The data are representative of three independent experiments.