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. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: Curr Biol. 2009 Jun 18;19(14):1200–1205. doi: 10.1016/j.cub.2009.05.050

Figure 1.

Figure 1

Pipe dependent labeling of vitelline membrane proteins

(A) Autoradiogram of ovarian extracts from [35S]Na2SO4-fed pipe mutant (left lane), wild-type (middle lane) and Pipe overexpressing (right lane) females. Note the presence of the 120 kDa band associated with Pipe overexpression. Maternal genotypes are indicated at top. (B) The 120 kDa band is absent from ovarian extracts of [35S]Na2SO4-fed VmlEPgy2 /Vml EPgy2 mutant females that over-expressed Pipe (right lane). The heavily labeled bands between 49 and 64 kDa in all lanes in panels A and B correspond to yolk proteins, which are known to undergo sulfation [32]. (C) Detection of Pipe dependent labeled species from purified eggshell/extracellular matrix preparations of ovarian extracts [14] from [35S]Na2SO4-fed pipe mutant (left lane), wild-type (middle lane), and Pipe over-expressing females (right lane).