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. 2008 Apr 28;17(15):2357–2369. doi: 10.1093/hmg/ddn136

Figure 3.

Figure 3.

(A) Immunoblotting demonstrating transgene expression in cultured keratinocytes isolated from transgenic mice expressing FLAG-progerin and FLAG-wild-type lamin A. Blots were probed with mouse anti-FLAG (FLAG) and mouse anti-actin (ß-actin) antibodies. Lanes numbered 361, 571, 777 and 796 are from two transgenic mouse lines containing the FLAG-progerin transgene (K14-progerin) and lanes numbered 763, 787, 660 and 851 are from two transgenic mouse lines with the FLAG-wild-type human lamin A transgene; wt indicates proteins extracted from skin of a non-transgenic littermate. (B) Confocal immunofluorescence micrographs of cultured mouse keratinocytes expressing FLAG-progerin (K14-progerin; panels a and d), FLAG-wild-type human lamin A transgene (K14-lamin A; panels b and e) and a non-transgenic littermate (WT littermate; panels c and f). Panels a, b and c show double labeling with anti-FLAG (green) and anti-keratin 14 (red) primary antibodies. For panels d, e and f, cells were labeled with anti-FLAG (green) and anti-lamin A/C (red) antibodies and overlay (yellow) of signals are shown. Bar: 10 µm.