Figure 5:

Genistein (Gen)-induced interactions between ERα and IGF-IR in human uterine LM cells.

Interactions between ERα and IGF-IR were determined by using immunoprecipitation (IP) as described in Materials and Methods. LM cells were treated with 0.3% DMSO (Control) at 0 min, or 1 µg/ml of Gen at 5, 10 and 15 min, or 1 µM ICI 182,780 (ICI) for 10 min, or Gen in combination with 1 µM ICI 182,780 (ICI+Gen) for 10 min. The blots presented are representative examples of experiments that were performed at least three times with repetitive results. Molecular weight markers are indicated on the right of the blot. The histograms indicated below are the quantitative representation after densitometry of data (mean ± SD) (n= 3) of three independent experiments. Values of ERα band densities in LM cells were divided by the vehicle control (set at 1), *P< 0.05 versus vehicle control containing 0.3% DMSO. **P< 0.05 versus Gen-treated at 10 min.