Table 2.

FA released from P815s under varying conditions^a

	Effector cells or mediator used in experiment
	Tc1 CTLs	Tc2 CTLs	Cytotoxic granule extract
Control live P815sb	1.80%	2.00%	6.00%
2× freeze–thawc	4.16%	4.02%	N/D
8:1 CTL:P815d	4.97%	5.18%	N/D
1/20 granule additione	N/D	N/D	9%

^aThe FAs were extracted from the cell-free supernatants at the end of 4 h incubations of the cells.

^bP815 were labeled with [³H]-oleic acid and allowed to incubate alone for 4 h.

^cRelease of FAs by P815 cells after 2× freeze–thaw cycles.

^dRedirected lysis was at an effector to target ratio of 8 to 1. For Tc1 cells, 83% of the P815 cells were killed and for Tc2 cells, 67% of the P815 cells were killed. The data are representative of two similar experiments.

^eCytotoxic granule extract (0.0574 μg ml⁻¹) was added to P815 targets that were treated with 5 mM 2-deoxyglucose, 1.5 mM NaN₃ and 1 mM KCN to increase tumor cell sensitivity to perforin. The lysis by granule extracts was 74%, respectively.