The effect of middle age-onset CR and antioxidant supplementation on the expression of biomarkers of cerebellum aging. Relative amount of mRNA compared to young control is shown for each group. (A) C4, (B) C1qa, (C) Ctss, (D) Lzp-s, (E) Gfap. C5, 5-month-old control; C30, 30-month-old control; CR, caloric restriction; LA, α-lipoic acid; CQ, coenzyme Q10; RE, resveratrol; CU, curcumin; LY, lycopene; AC, acetyl-L-carnitine; AS, astaxanthin; TP, tempol. Lines above/below bars indicate standard error. The one-way ANOVA test showed significant p-value (P < 0.01) in all genes tested. * Significantly different from C30 (P < 0.05).