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. Author manuscript; available in PMC: 2009 Aug 27.
Published in final edited form as: Methods Mol Biol. 2007;356:353–365. doi: 10.1385/1-59745-217-3:353

Table 1.

Recommended Volumes of 20 μM Stock siRNA and 1 μg/μL Lipofectamine 2000 for Different Final Concentrations of siRNA

siRNA final concentration
Culture format 40 (nM) 80 (nM) 120 (nM)
24-well (μL/well) 1.25 2.5 3.75
96-well (μL/well) 0.25 0.5 0.75