Table 1.
Accuracy and Precision of the LC–APCI/MS Method, Based on Recovery of Known Amounts of Phylloquinone Added to Plasma Samples (n = 3)
| unlabeled phylloquinone |
deuterium-labeled phylloquinone |
|||
|---|---|---|---|---|
| spiked concentration (nmol/L) | mean recovery (%)a | RSD (%)b | mean recovery (%) | RSD (%) |
| 0.5 | 97.8 ± 15.1 | 15.4 | 92.2 ± 7.2 | 7.8 |
| 1.0 | 92.4 ± 3.2 | 3.5 | 87.7 ± 7.7 | 8.8 |
| 2.0 | 98.8 ±4.3 | 4.4 | 96.0 ± 7.7 | 7.9 |
| 4.0 | 97.7 ± 3.1 | 3.1 | 93.5 ± 9.0 | 9.6 |
| 8.0 | 91.2 ± 1.2 | 1.4 | 99.6 ± 8.8 | 8.7 |
| 16.0 | 95.9 ± 7.1 | 7.3 | 97.6 ± 2.6 | 2.7 |
| 32.0 | 103.2 ± 2.8 | 2.7 | 102.0 ± 3.4 | 3.3 |
a
Mean percent relative recovery (mean % ± SD) represents the accuracy of the method.
b
Percent relative standard deviation (RSD) represents the precision of the method.