Figure 1.

A model of endocytic recycling at the tip region in A. oryzae. (A) In endocytosis-deficient hyphae, large invaginated structures are stained with Calcofluor White, which binds to chitin, one of major cell wall components in filamentous fungi (arrow). Bar, 5 µm. (B) In this figure, motor proteins, such as kinesins and dynein, which drive early endosomes moving on microtubules bidirectionally, are omitted. Components, which should be re-transported to the tip region, are internalized from the subapical tip region of plasma membrane where endocytosis actively occurs, to ERC, and secretory vesicles are subsequently transported to Spitzenkörper. Although there is no evidence, ERC possibly exists not only in the apical region but also, to some extent, in the subapical and basal region (the latter two strategies are technically represented by the opposite of the highlighted arrows shown above).