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. 2009 Sep 9;4(9):e6957. doi: 10.1371/journal.pone.0006957

Figure 4. Interaction of Synthetic Hemozoin Crystals with DNA or Proteins.

Figure 4

(A) 100 µg of nHZ, rcHZ or scHZ were left untreated or were incubated with 1 µg of DNA extracted from B10R Mφ or from promastigote cultures of Leishmania major. After washes, recovered pellets were resuspended in sample loading buffer and run in a 1% agarose gel containing 0.5 µg/ml ethidium bromide. As positive controls for DNA contents, 1 µg of Mφ or parasite DNA were loaded. (B) SDS-PAGE and silver staining of 100 µg of sHZ crystals (nHZ, rcHZ and scHZ) incubated in absence or in presence of DMEM containing either 10% FBS or 5 µg of BSA. As positive controls for protein contents, 2.5% FBS or increasing concentrations of BSA (50, 500 and 1000 ng/ml) were loaded.