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. 2009 Apr 17;63(6):1163–1172. doi: 10.1093/jac/dkp129

Figure 2.

Figure 2

Microscopic examination of live and heat-killed B. burgdorferi strain N40 clone D10/E9 after Hoechst/Sytox Green double staining. The samples were centrifuged after incubation with the nucleic acid dyes, washed once with PBS and mounted in 1:1 PBS/glycerol mixture. The cover glasses were sealed with nail polish to limit evaporation. DIC provision in the Nikon 80i fluorescence microscope was used to observe total spirochaetes present in any field of view. DAPI (for Hoechst 33342 staining) and FITC (for Sytox Green) filters were used. Total spirochaetes were observed by Hoechst staining and physiologically compromised spirochaetes by Sytox Green staining by using an Apo-Plan TIRF ×100 magnification objective. This figure appears in colour in the online version of JAC and in black and white in the print version of JAC.