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Summary of specific activity measurements for Bgp and Pfs
| Enzyme | Compound | Specific activity (U/mg) | Relative activity (%) |
|---|---|---|---|
| Bgp | MTA | 4.1 | 100 |
| SAH | 3.1 | 76 | |
| pNH2PhTA | 0.2 | 5 | |
| pNO2PhTA | 0.82 | 20 | |
| FMA | 0 | 0 | |
| BnT-DADMe-ImmA | 0 | 0 | |
| Pfs | MTA | 4.4 | 100 |
| SAH | 3.7 | 84 | |
| pNH2PhTA | 0.024 | 0.6 | |
| pNO2PhTA | 2.0 | 45 | |
| FMA | 0 | 0 | |
| BnT-DADMe-ImmA | 0 | 0 |
Enzyme activities were tested using the UV spectrophotometric assay (275 nm) and either 100 µM substrate (MTA and SAH) or 100 µM substrate analogue (pNH2PhTA, pNO2PhTA, FMA and BnT-DADMe-ImmA).
