Figure 2.

Substrate induced conformational changes upon ubiquitin binding to DUBs. A) a loop occluding the active must be displaced to allow substrate binding and access to the active site. B) A larger domain occluding the active site must be displaced upon substrate binding or association with a scaffold protein. C) The binding of the substrate induces a conformational change in the catalytic triad allowing it to achieve a catalytically competent geometry.