Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Apr;11(4):394–397. doi: 10.1128/jcm.11.4.394-397.1980

Evaluation of rapid carbohydrate degradation tests for identification of pathogenic Neisseria.

D J Pizzuto, J A Washington 2nd
PMCID: PMC273411  PMID: 6768769

Abstract

A total of 156 clinical isolates were tested by the modified rapid fermentation test, the BACTEC Neisseria differentiation kit, and the cystine-Trypticase agar method. The modified rapid fermentation test and BACTEC methods accurately identified at least 95% of 101 strains of N. gonorrhoeae tested and at least 91% of 45 strains of N. meningitidis tested within 4 h. Overall, the cystine-Trypticase agar method was the most accurate (97%) but required as long as 48 h of incubation. The data presented appear to show that rapid carbohydrate degradation tests can provide reliable and specific identification results.

Full text

PDF
394

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum P. C., Lawrence R. B. Comparison of three methods for identification of pathogenic Neisseria species. J Clin Microbiol. 1979 May;9(5):598–600. doi: 10.1128/jcm.9.5.598-600.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnham M., Glynn A. A. Identification of clinical isolates of Neisseria gonorrhoeae by a coagglutination test. J Clin Pathol. 1978 Feb;31(2):189–193. doi: 10.1136/jcp.31.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beck A., Fluker J. L., Platt D. J. Neisseria meningitidis in urogenital infection. Br J Vener Dis. 1974 Oct;50(5):367–369. doi: 10.1136/sti.50.5.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown W. J. A comparison of three fermentation methods for the confirmation of Neisseria gonorrhoeae. Health Lab Sci. 1976 Jan;13(1):54–58. [PubMed] [Google Scholar]
  5. Brown W. J. Modification of the rapid fermentation test for Neisseria gonorrhoeae. Appl Microbiol. 1974 Jun;27(6):1027–1030. doi: 10.1128/am.27.6.1027-1030.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Danielsson D., Kronvall G. Slide agglutination method for the serological identification of Neisseria gonorrhoeae with anti-gonococcal antibodies adsorbed to protein A-containing staphylococci. Appl Microbiol. 1974 Feb;27(2):368–374. doi: 10.1128/am.27.2.368-374.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Devine L. F., Hagerman C. R. Relationship of Serogroups of Neisseria meningitidis I. Microagglutination, Gel Diffusion, and Slide Agglutination Studies of Meningococcal Antisera Before and After Absorption with RAS-10 Strain of Meningococci. Infect Immun. 1970 Mar;1(3):226–231. doi: 10.1128/iai.1.3.226-231.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Faur Y. C., Weisburd M. H., Wilson M. E. Carbohydrate fermentation plate medium for confirmation of Neisseria species. J Clin Microbiol. 1975 Mar;1(3):294–297. doi: 10.1128/jcm.1.3.294-297.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Faur Y. C., Weisburd M. H., Wilson M. E. Isolation of Neisseria meningitidis from the Genito-urinary tract and anal canal. J Clin Microbiol. 1975 Sep;2(3):178–182. doi: 10.1128/jcm.2.3.178-182.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fiumara N. J., Wise H. M., Jr, Many M. Gonorrheal pharyngitis. N Engl J Med. 1967 Jun 1;276(22):1248–1250. doi: 10.1056/NEJM196706012762208. [DOI] [PubMed] [Google Scholar]
  11. Flynn J., Waitkins S. A. A serum-free medium for testing fermentation reactions in Neisseria gonorrhoeae. J Clin Pathol. 1972 Jun;25(6):525–527. doi: 10.1136/jcp.25.6.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hampton K. D., Stallings R. A., Wasilauskas B. L. Comparison of a slide coagglutination technique with the Minitek system for confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1979 Sep;10(3):290–292. doi: 10.1128/jcm.10.3.290-292.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holmes K. K., Counts G. W., Beaty H. N. Disseminated gonococcal infection. Ann Intern Med. 1971 Jun;74(6):979–993. doi: 10.7326/0003-4819-74-6-979. [DOI] [PubMed] [Google Scholar]
  15. Iqbal Y. Gonococcal tonsillitis. Br J Vener Dis. 1971 Apr;47(2):144–145. doi: 10.1136/sti.47.2.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kellogg D. S., Jr, Turner E. M. Rapid fermentation confirmation of Neisseria gonorrhoeae. Appl Microbiol. 1973 Apr;25(4):550–552. doi: 10.1128/am.25.4.550-552.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morse S. A., Bartenstein L. Adaptation of the Minitek system for the rapid identification of Neisseria gonorrhoeae. J Clin Microbiol. 1976 Jan;3(1):8–13. doi: 10.1128/jcm.3.1.8-13.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morse S. A., Stein S., Hines J. Glucose metabolism in Neisseria gonorrhoeae. J Bacteriol. 1974 Nov;120(2):702–714. doi: 10.1128/jb.120.2.702-714.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pollock A. A., Holzman R. S. Letter: Neisseria catarrhalis endocarditis. Ann Intern Med. 1976 Aug;85(2):206–207. doi: 10.7326/0003-4819-85-2-206. [DOI] [PubMed] [Google Scholar]
  20. Pollock H. M. Evaluation of methods for the rapid identification of Neisseria gonorrhoeae in a routine clinical laboratory. J Clin Microbiol. 1976 Jul;4(1):19–21. doi: 10.1128/jcm.4.1.19-21.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. REYN A. LABORATORY DIAGNOSIS OF GONOCOCCAL INFECTIONS. Bull World Health Organ. 1965;32:449–469. [PMC free article] [PubMed] [Google Scholar]
  22. Reddick A. A simple carbohydrate fermentation test for identification of the pathogenic Neisseria. J Clin Microbiol. 1975 Jul;2(1):72–73. doi: 10.1128/jcm.2.1.72-73.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Reyn A. Recent developments in the laboratory diagnosis of gonococcal infections. Bull World Health Organ. 1969;40(2):245–255. [PMC free article] [PubMed] [Google Scholar]
  24. Shtibel R., Toma S. Neisseria gonorrhoeae: evaluation of some methods used for carbohydrate utilization. Can J Microbiol. 1978 Feb;24(2):177–181. doi: 10.1139/m78-030. [DOI] [PubMed] [Google Scholar]
  25. Strauss R. R., Holderbach J., Friedman H. Comparison of a radiometric procedure with conventional methods for identification of Neisseria. J Clin Microbiol. 1978 May;7(5):419–422. doi: 10.1128/jcm.7.5.419-422.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wilson H. D., Overman T. L. Septicemia due to Neisseria lactamica. J Clin Microbiol. 1976 Sep;4(3):214–215. doi: 10.1128/jcm.4.3.214-215.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yong D. C., Prytula A. Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1978 Dec;8(6):643–647. doi: 10.1128/jcm.8.6.643-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES